In each program, the Cat in the Hat and his friends Sally and Nick go on a science adventure such as shrinking to bee-size to explore a hive and discover how honey is made; flying with birds to discover how and why they migrate; diving inside flowers to find out more about the animals that depend on them to live; or taking a snowcat to the Arctic to explore freezing and melting. Guided by the Cat, the children figure things out by engaging in science inquiry. They ask questions, make observations, make predictions, plan investigations, collect data, make discoveries, and generate and discuss ideas about how the world works.

Each half-hour television episode consists of two 11-minute animated adventures, along with corresponding short animated clips. Each adventure revolves around a specific science concept such as bird migration or animal camouflage. The animated clips feature songs and rhymes, interesting science facts, humorous science explorations by Thing One and Thing Two, and interviews of animals by Sally and Nick.

The Cat in the Hat Knows a Lot About That!™ is voiced by award-winning actor Martin Short and produced by Portfolio Entertainment Inc. and Collingwood O’Hare Productions, in association with Dr. Seuss Enterprises, Random House Children’s Entertainment, Treehouse, and PBS KIDS.