- How has the gel changed?
(there are bubbles at the electrodes, the gel has softened,
the food coloring has spread into the gel)
- Did the coloring spread out evenly in all directions?
(No. The color broke up into connected smears that spread
varying distances from the well)
- Did the food coloring change shades?
(Yes - The green separated out into regions of blue and
yellow. The yellow traveled the furthest.)
- Did the different food colors separate into the same bands?
(No - each color separated into its own smear of component
of DNA have an overall negative charge. If you were to perform
electrophoresis on DNA, at which side of the well would you
observe the formation of DNA bands? Why? (On the positive
side of the well since opposite charges attract.)
examining the separation bands of DNA, you discover that the
smaller fragments of DNA travel the greatest distance from
the well. Why? (Smaller size particles make fewer collisions
with the molecules that form the gel matrix. With fewer collisions,
their movement is less affected than the migration of larger
(more frequently colliding) particles.)