Teachers Powered by teachers'domain

Forensic DNA Analysis

  • Teacher Resource
  • Posted 09.26.03
  • NOVA

This video segment from NOVA: "The Killer's Trail" follows a team of experts as they investigate the forensic evidence from the 1954 murder of Marilyn Sheppard, one of the most famous unsolved crimes in U.S. history.

Permitted use: Download Download

NOVA Forensic DNA Analysis
  • Media Type: Video
  • Running Time: 6m 29s
  • Size: 19.3 MB
  • Level: Grades 9-12

  • Log in to Teachers' Domain to download, share, rate, save, and match to state standards.

Source: NOVA: "The Killer's Trail"

This resource was adapted from NOVA: "The Killer's Trail."


DNA fingerprinting, also called forensic DNA analysis, is considered by many to be the police investigator's secret weapon, a means of building cases or reanalyzing crimes using tiny bits of cryptic evidence. Indeed, since 1986, when DNA evidence first entered the courtroom, the technique has aided in the prosecution or defense of hundreds of cases, and in the exoneration of dozens of people wrongly convicted.

As the acceptance of DNA evidence in the courtroom has grown, so has its importance, for the simple reason that physical evidence linking suspects to crimes is often very sparse. Sometimes the weight of an entire case -- even the life of the individual on trial -- rides on just a few drops of blood or strands of hair. But as the reinvestigation of the 1954 murder of Marilyn Sheppard shows, the quality of evidence can dramatically affect the amount of information that evidence provides.

In general, forensic DNA analysts compare the genetic makeup of tissue samples in search of similarities and differences among them. They do this not by comparing all of the DNA contained in each cell, but instead by marking a small number of segments and then checking for the presence or absence of those segments in each sample.

One of the most common techniques, called variable number tandem repeat (VNTR) analysis, isolates DNA segments that all have the same sequence of repeating letters (ATCATCATC, for example). It organizes these repeating segments according to length, marks segments of a few different lengths, and then compares samples based on the presence or absence of same-length segments. Two samples that have as many as ten or twelve of these segments in common have very little chance -- one in several million -- of being from two different people. Because VNTR analysis relies on samples that contain relatively long strands of intact DNA, the technique usually cannot be used to analyze tissues as old as the bloodstains found in the Sheppard house. For decades-old samples, forensic analysts use instead a variety of polymerase chain reaction (PCR) techniques, including DQA1. The DQA1 analysis focuses on one tiny segment of the genome. This segment, the DQA locus, holds eight alleles, each of which codes for a different protein, and six of which can be marked and used for forensic analysis.

In a DQA1 analysis, lab technicians compare samples with regard to the presence or absence of these six DQA alleles. However, because the alleles come in a small number of combinations (just forty-two), it's possible that a person could match a sample from a crime scene without being the source of that sample. For this reason, DQA1 is typically used only to rule out suspects, and not implicate them.

Questions for Discussion

  • How was DNA evidence used to prove that Dr. Sam Sheppard did not murder his wife? Why wasn't this evidence used when the case first went to court?
  • Why do you think the DQA1 test was chosen for DNA analysis in this case, instead of another, more powerful genetic test?
  • If the blood trail left at the murder scene wasn't Marilyn's or Sam's, whose blood might it have been?
  • If you were a juror on this trial, would you be convinced by the DNA evidence?

Resource Produced by:

					WGBH Educational Foundation

Collection Developed by:

						WGBH Educational Foundation

Collection Credits

Collection Funded by:

						National Science Foundation

Related Resources

  • Forensics on Trial

    Virtual autopsies, 3-D fingerprints, and digital crime scenes are making crime-solving into a more precise science.

  • Forensics

    Find out how DNA profilers analyze bits of tissue to identify human remains. From NOVA: "Lost on Everest" We...

  • Cracking Your Genetic Code

    We are on the brink of a new era of personalized, gene-based medicine. Are we ready for it?

  • Extracting Mummy DNA

    This video segment explains how a DNA sample is taken from a 5,000-year-old mummy and what we can learn from it.

  • Create a DNA Fingerprint

    Assemble a virtual DNA fingerprint and use it to identify the culprit in a hypothetical crime.